几种动物的抗-HBs应答观察

Observation of Antibody Response to HBs Antigen in Several Animal Species

许多动物种属(包括一些家畜和家禽)中存在低滴度抗-HBs,推测其产生原因可能与HBAg有关,或由于动物暴露于与 HBsAg 有共同表位的环境抗原,诱导可引起交叉反应的抗体。本文用 HBsAg 阳性、HBeAg 阴性的 HBV 感染患者血清免疫豚鼠、鸡和鹅各 2只,均产生抗-HBs应答,其抗-HBs 活性可被纯化 HBsAg 中和,证明此抗-HBs 是特异性的。采用 SPRIA 检测,豚鼠抗-HBs 滴度高于 1:800,而鸡和鹅虽经多次加强免疫,滴度仅达 1:10～1:100,表明鸡、鹅对HBsAg 的反应性低,仅引起较弱的抗-HBs 应答,从而证实动物 HBsAg 暴露是产生低滴度抗-HBs的原因之一。另外,观察免疫后动物血清,除 HBsAg 阳性(RPHA≥1:128)外,未出现其它 HBV感染标志,无显著的生化和病理改变,肝脏 HBsAg 阴性,提示动物未经历 HBV 活性复制。

The guinea-pigs, chickens and geese immunized with human positive HBsAg serum yielded an anti-HBs response. The antibody activation can be neutralized by purified HBsAg preparation. The anti-HBs titre generated in chickens and gooses only were 1: 10. Some scholars have found that low titre anti-HBs is present in many animal species. Our studies proved that animal HBsAg exposure is one of the causes producing low titre anti-HBs. HBV active replication markers hadn't been detected in all experimential animals.