益气养阴祛瘀中药对干燥综合征自发性模型非肥胖糖尿病小鼠的治疗作用及机制

Effects of Notifying Qi Nourish Yin and Remove Stasis Herbs on Spontaneous Models of Sjogrens Syndrome in the Non-obese Diabetic Mice

[目的]探讨益气养阴祛瘀中药对非肥胖糖尿病(NOD)自发性干燥综合征(SS)样小鼠的疗效及作用机制。[方法]将45只8周龄NOD自发性SS样小鼠随机分为对照组、中药组和西药组灌胃治疗12周,分别检测各组治疗前、后小鼠不同时间点(9、15和20wk)的唾液分泌量,检测各组小鼠血清的IgG、IgA、C3,比较各组小鼠颌下腺、胸腺和脾脏指数情况,并观察各组颌下腺组织病理学改变;同时采用RT-PCR法检测各组颌下腺组织BAFF和NF-κB mRNA表达水平。[结果]中药组对NOD小鼠的唾液分泌量、颌下腺指数和颌下腺炎性浸润较对照组均有不同程度地改善(P<0.05或P<0.01);其疗效也更优于单用羟氯喹的西药组(P<0.05)。中药组能明显下调NOD小鼠颌下腺BAFF mRNA的表达水平,能使NOD实验小鼠在治疗后外周血IgG和C3水平改善(P<0.05或P<0.01)。[结论]益气养阴祛瘀中药对NOD小鼠SS具有有效的治疗作用,将为治疗SS提供一种新的治疗方法,而其作用机制与下调BAFF分子表达密切相关。

[Objective]To investigate the effects of Notifying Qi Nourish Yin and Remove Stasis herbs on Spontaneous Models of Sjogrens Syndrome in the non-obese diabetic（NOD） mice and its pharmacological mechanism.[Methods]45 female NOD mice（8-week-old） were randomly divided into three groups：Chinese medicine,hydroxychloroquine（HCQ）and normal saline（NS）group.One week later,they were administered intragastrically in No-tifying Qi Nourish Yin and Remove Stasis herbs,HCQ and NS respectively,and were orally administered for 12 weeks.Three mice from each group were sacrificed at the age of 9,15 and 20 weeks.The saliva flow,serum and submandibular glands were collected at these time points.Histological changes of submandibular glands were examined by HE staining.The levels of serum IgA,IgG and C3 were detected.The expression of BAFF and NF-κB mRNA in the salivas were also detected by PT-PCR.[Results]At the end of the study,the salivary flow was significantly increased,the autoantibodies in serum,the expression of BAFF mRNA and histological grading of salivary tissue were significantly lower in the Chinese medicine treatment groups（P0.05 or 0.01）.Compared with the HCQ group,the Chinese medicine group was better.[Conclusion]Notifying Qi Nourish Yin and Remove Stasis herbs can relieve the dry mouth symptom and change the pathological of salivary tissue of Sjogrens Syndrome in the NOD mice.Its mechanism may be associated with inhibition of mRNA expression of BAFF.