定量ELISA法检测健康人血浆抗呼吸道合胞病毒IgG抗体的可行性研究

Feasibility study on a quantitative ELISA for detecting IgG antibody against respiratory syncytial virus in healthy human plasma

目的确认定量ELISA法检测健康人血浆抗呼吸道合胞病毒（respiratory syncytial virus,RSV）IgG抗体的可行性。方法采用定量ELISA法检测健康人血浆，对初检的抗-RSV阳性血浆（抗-RSV效价〉11U／ml）进行复检和中和试验。结果检测3041份血浆，初检和复检的血浆抗-RSV阳性率分别为5．4％和4．7％。定量ELISA法的特异性和精密性均良好，抗-RSV阳性血浆的有效检出率为87．8％。结论定量ELISA法可用于健康人血浆抗RSVIgG抗体的大规模筛检。

Objective To identify feasibility of a quantitative ELISA for detecting IgG antibody against respiratory syncytial virus （RSV） in healthy human plasma. Methods The quantitative ELISA was used to detect anti-RSA IgG in healthy human plasma. The positive plasma samples （ anti-RSV titer 〉 11 U/ml） were detected again by the quantitative ELISA. A neutralization assay was carried out meanwhile. Results In 3041 plasma samples detected, anti-RSV positive rates of initial and repeat detection were 5.4% and 4. 7% , respectively. The quantitative ELISA had good specificity and precision. The efficient detection rate of antiRSV positive plasma was 87.8%. Conclusion The quantitative ELISA can be applied to mass screening of anti-RSV IgG antii）ody in healthy human plasma.