Smo蛋白在肝癌和肝硬化中的表达及临床意义

Expression of Smo Protein in Hepatocellular Carcinoma and Liver Cirrhosis and its Clinical Significance

目的:研究Smoothened(SMO)在肝癌和肝硬化中的表达及临床意义。方法:选取组织标本后,运用石蜡包埋,切片后,HE染色,构建组织芯片,免疫组织化学和原位杂交方法检测Smo蛋白在肝癌和肝硬化中的表达。结果:Smo蛋白在肝癌细胞浆、良性肝肿瘤组织细胞浆、肝硬化组织中强染色,在正常组织中无染色。并且在典型肝硬化中强染色,在中度肝硬化中弱阳性。结论:Smo蛋白表达与肝癌的发生有关,Smo基因的高表达可能激活某种机制而参与诱导肝癌的发生。可能是通过异常激活Sonic hedgchog信号通路,从而诱导肝癌的发生与发展。

Objective： To study the expression of Smoothened（SMO） in hepatocellular carcinoma and liver cirrhosis and its clinical significance.Methods： The selected tissue specimens were embedded with paraffin,and HE stained after sectioned.Constructed tissue microarrays.Use immunohistochemistry and in situ hybridization method to detect the Smo protein expression in hepatocellular carcinoma and liver cirrhosis.Results： Smo protein had strong staining in cytosol of hepatocellular carcinoma,benign liver tumor tissue and liver cirrhosis tissue,but no staining in the normal tissue.And it had a strong staining in typical liver cirrhosis,and is weak positive in moderate liver cirrhosis.Conclusion： The expression of Smo protein is related to the occurrence of hepatocellular carcinoma.The higher expression level of Smo gene may activate a mechanism involved in the induction of hepatocellular carcinoma.It induces the occurrence and development of hepatocellular carcinoma probably by abnormally activating the Sonic hedgchog signaling pathway.