早期激素性股骨头坏死模型的建立

Construction of the Model of First-Stage Steroid-Induced Femoral Head Necrosis in Rabbits

目的:建立早期股骨头坏死模型,为研究其发病机制及合理治疗方法的提供可靠的模型基础。方法:用单一剂量脂多糖LPS(10μg/kg)联合三次甲强龙MPS(10 mg/kg)注射,每次间隔24h,诱导建立兔早期股骨头坏死模型,通过影像学及组织病理性学方法评估模型建立情况。结果:4周后,模型组死亡1例,模型组16例X线检查未见异常表现,2例X线提示股骨头密度不均,未出现股骨头塌陷,18例HE染色示骨细胞空骨陷窝增多,脂肪细胞体积变大,数量增多。结论:用脂多糖(LPS)联合甲强龙可成功诱导建立兔早期股骨头坏死模型,模型成功率高、死亡率低。

Objective： To construct the model of first-stage steroid-induced osteonecrosis in rabbits and prepare to seek for pathogenesis and rational treatment of first-stage osteonecrosis of femoral head.Methods： We constructed the model with lipopolys-accharide（signal-dose 10ug/kg） plus methylprodnisolone（10mg/kg,three times every 24h internals）.The osteonecrosis model was evaluated by X-ray examination and hematoxylin and eosin stain.Results： In model group,two samples were featured with uneven density of femoral head in X-ray photograph.There were 18 samples with increased numbers of empty bone lacunae and larger quantity of fat cells compared with normal grop under light microscopy and histological.Conclsion： The animal model was induced by LPS plus MPS,with a high success rate and low mortality.