九项生化试验对弧菌科10种致病菌鉴定的探讨

A Preliminary Study on Identification of Vibrio (genera, species)by the Nine Kinds of Biochemical Reaction

目前弧菌科(属种)的鉴定,主要依靠生化性状的试验。本文根据Rypka等(1967)报道的选择最佳鉴别性状的推理方法,从众多的生化试验项目中进行统计学分析优选出九项(即精氨酸、赖氨酸、V-P、阿拉伯糖、甘露糖、蔗糖、无盐胨水、6％盐胨水、10％盐胨水等),组成生化鉴定系统与常规生化试验(11～16项)对疑似弧菌科细菌318株进行鉴定,符合率可达100％;试验结果表明,用最少的生化试验项目,达到了对弧菌科临床常见弧菌的鉴定,既节省了时间,又节约了经费。本次试验将九项生化试验培养基制成后分装安瓿,做成生化鉴定药盒,操作简单,使用方便,对基层实验室有推广价值。

Nowadays, the biochemical reaction was also the main method for identification of Vibrio. In this paper accor-ding to Rypka's method authors chose out nine from various kinds of biochemical reaction to identificate Vibrio. It included NO3 reduction, lysine decarboxylase, Voges-Proskauer test, D-Arabi-nose, Mannose, Sucrose and Peptone water containing 0%,6%,10% of NaCl. Using the nine reaction to identificate 318 strains of Vibrio-like bacteria and compare with routine method(11-16 kinds) of biochemical reaction,the corresponding rate was 100%. The results showed that the nine reactionsystem may be used for simple identification of Vibrio.