石藤胶囊对佐剂性关节炎大鼠OPG、RANKL表达的影响

The Effect of Shiteng Capsules on Expression of OPG and RANKL in Rats with Adjuvant-Induced Arthritis

[目的]观察石藤胶囊对佐剂性关节炎(A A)大鼠血清及关节组织核因子κB受体激活配体(RANKL)、骨保护素(OPG)的影响。[方法]采用弗氏完全佐剂诱导的大鼠A A模型。Wistar大鼠32只,随机分为模型组、石藤胶囊组、雷公藤组,正常对照组各8只。酶联免疫吸附剂测定(ELISA)法测定大鼠血清中核因子KB受体激活配体(RANKL)、骨保护素(OPG)的水平,免疫组织化学法检测大鼠关节中OPG、RANKL的表达。[结果]石藤胶囊可以明显降低A A大鼠血清和关节中的RANKL水平,提高血清和关节中的OPG水平。[结论]石藤胶囊能够通过抑制破骨细胞通道对AA发挥防治作用。

[Objective] To observe the effect of Shiteng capsule to RANKL and OPG in blood serum of rats with adjuvant-induced arthritis. [ Methods] 32 Wistar rats were divided randomly and averagely into 4 groups as following： model, Shiteng capsules, Tripterygium wilfordii and normal. The model was induced by Freund＇s complete adjuvant. The RANKL and OPG were detected by ELISA in blood serum of rats and the expression of OPG and RANKL were detected by immunohistochemical method in rats＇ joints. [Results] Shiteng capsules can cut down the RANKL level in rats＇ serum and joints with AA obviously, and boost the OPG level in serum and joints. [Conclusion] Shiteng capsules can bring into full play preventive and therapeutic effect to AA by refraining osteoclast CP.