EB VCA—IgA和EB—DNA检测对儿童传染性单核细胞增多症和鼻咽癌诊断价值的比较

The Compare of the Diagnostic Value of EB VCA-IgA and EB-DNA Detection for Infectious mononucleosis and nasopharyngeal carcinoma

目的探讨检测血清中EBVCA—IgA抗体与EB—DNA在儿童传染性单核细胞增多症和鼻咽癌中的诊断意义。方法采用ELISA方法和荧光定量PCR方法分别检测60例儿童传染性单核细胞增多症患者组和对照组，60例未经治疗的鼻咽癌患者组和对照组EBVCA—IgA抗体和EB—DNA，分别比较其阳性检出率来探讨他们对两种疾病的诊断价值。结果EBVCA—IgA抗体在儿童传染性单核细胞增多症患者组和对照组中的阳性检出率差异无显著性意义，在鼻咽癌患者组和对照组中的阳性检出率差异有显著性意义。EB—DNA检测在两种疾病患者组和对照组中的检出率差异均有显著性意义。结论VCA—IgA抗体检测对儿童传染性单核细胞增多症诊断意义不高，在鼻咽癌中有重要诊断意义。EB—DNA检测对两种疾病均有重要诊断意义，可作为诊断EBV感染最有效的方法之一。

Objective To investigate the application of Epstein-Barrvirus（EBV） VCA-IgA （EB virus capsid antigen-IgA,VCA-IgA） antibody and EB-DNA（Epstein- Barr vires DNA,EB-DNA） in diagnosis of Infectious mononucleosis （IM）children and nasopharyngeal carcinoma（NPC）. Methods EB VCA-IgA antibody and EB-DNA of 60 children with IM and 60 healthy controls, 60 untread patients with NPC and 60 healthy controls were checked by enzyme-linked immnunosorbent assay（ELISA） and fluorescence quantitative polymerase chain reaction（PCR）method. The positive rates were compared to study their clinic value for diagnosis of these two diseases. Results The positive rate of EB VCA-IgA antibody detection showed no significant difference between the IM children and the control group, while it showed significant difference between the NPC patients and the control group. The positive rates of of EB-DNA showed significant between the patients and the controls in both two diseases. Conclusion The EB VCA-IgA antibody detection is a useful method for the diagnosis of NPC, but not for IM. The EB-DNA detection is a useful method for both the diagnosis of NPC and IM. It could be the most effective method for diagnosing EBV infection.