离子色谱法同时测定尿碘和尿氟的探讨

Simultaneous determination of urinary iodine and urinary fluoride by ion chromatography

目的建立测定人体尿碘、尿氟离子浓度的离子色谱法。方法尿样经0.2μm滤膜过滤后,以8 mol/L碳酸钠水溶液作淋洗液,进样体积为50μl,流量1.8 ml/min.通过电导检测器测定,量程3μs,采用峰面积定量。结果该方法尿碘、尿氟回收率分别为95.0%～105.7%和98.7%～100.6%。RSD分别为2.30%和0.79%。最低检出限分别为I-30μg/L,F-100μg/L。线性范围I-30～300μg/L,r=0.998 0;F-100～1 500μg/L,r=0.999 3。结论该方法测定稳定、准确、快速,可用于实际样品的测定。

Objective To establish ion chromatography method for determination of human urinary iodine and urinary fluoride.Methods The urine sample was filtrated by 0.2 μm film with 8 mol/L sodium carbonate solution as eluent and injection volume of 50 μl and flow rate of 1.8 ml/min,conductivity detector was applied to detect with range of 3 μs,quantition was based on the peak area.Results Recovery rates of urine iodine and urine fluoride were 95.0%-105.7% and 98.7%-100.6%,RSDs were 2.30% and 0.79%,respectively.Minimum detection limits for I-and F-were 30 μg/L and 100 μg/L.The linear range of I-was 30-300 μg/L,r=0.998 0;F-for 100-1 500 μg/L,r=0.999 3.Conclusion This method is stable and accurate,and can be used for the determination of the actual samples.