透明质酸钠预防术后腹腔粘连的实验研究

EXPERIMENTAL STUDY OF SODIUM HYALURONATE IN PREVENTING POSTOPERATIVE ABDOMINAL ADHESION

目的 探讨透明质酸钠对术后腹腔粘连的预防作用。方法 将３８ 只大鼠随机分为Ａ、Ｂ、Ｃ ３ 组，于腹腔内损伤部位分别注入生理盐水、６ ％ 右旋糖酐４０ 和透明质酸钠（ 术康林１） ，术后第１４ 天处死动物，观察各组腹腔粘连情况。结果 ３ 组粘连分级差异有显著性意义（ Ｐ＜ ０ ．０１） 。光镜下见Ｃ 组炎症反应轻微，纤维增生不显著； 电镜下见Ｃ 组纤维细胞分泌胶原能力弱，间质细胞增生活跃。结论 透明质酸钠可减少术后腹腔粘连的程度，其作用优于右旋糖酐。

Objective To determine the effectiveness of sodium hyaluronate (SHA) in preventing intraperitoneal (IP) adhesion. Methods Thirty eight rats were randomly divided into A,B,C groups, normal saline, 6% Dextran 40 or SHA were applied on the present serosal injury respectively, during operation. Biopsy was taken on the 14th postoperative day. Results There were statistically significant differences in the extent of adhesion among three groups ( P <0.01). Mild inflammatory changes and less fibrous proliferation were found in group C by microscopy and decreased production of collagen (by fibroblast) and active mesothelial cells proliferation were observed in group C under electron microscope. Conclusion SHA appeares to reduce the extent of postoperative IP adhesion, which is more satisfactory than Dextran 40.