摘要
目的 建立稳定的肝硬变门脉高压症模型。方法 对大鼠在左肾静脉周围去血管化后皮下注射四氯化碳，然后观察肝脏组织形态学、门静脉系血管压力及分布、肝外门静脉系及侧支循环变化。结果 实验性肝硬变在形成过程中历经肝细胞变性坏死， 纤维组织增生及假小叶形成过程。实验第５ 周始， 门静脉压力显著高于对照组（ Ｐ＜ ０ ．０５） ；实验第１５ 周时，食管静脉管径显著大于对照组（ Ｐ＜ ０ ．０５） 。结论 大鼠左肾静脉周围去血管化后皮下注射四氯化碳所诱导的大鼠肝硬变门脉高压症模型简便、可靠，可广泛应用于门脉高压症的实验研究。
Objective To establish the reliable model of hepatocirrhosis with portal hypertension. Methods Carbon tetrachloride was subcutaneous injected after devascularization of the circumference of the left renal vein. The morphology of liver, the extrahepatic portal system and portcaval collateral circulation were observed. Results The liver of rats underwent degeneration and necrosis of hepatocytes, and the normal architecture of the liver lobules was replaced by pseudolobules, which consisted of regenerative hepatocytes and fibrous septa. After 5 weeks, the portal pressure was significantly higher than that of control ( P <0.05). The diameter of esophageal vein was significantly larger than that of control at 15th week ( P <0.05). Conclusion This technique is simple and reliable and can be applied for various experimental studies on portal hypertension.
出处
《中国普外基础与临床杂志》
CAS
2000年第1期22-24,共3页
Chinese Journal of Bases and Clinics In General Surgery
基金
国家自然科学基金!资助号39770207
