H-2K^b基因转移诱导小鼠心肌移植免疫耐受

Induction of myocardium transplantation tolerance by H-2K^b gene transfer in mice

目的探讨H-2Kb基因转移诱导小鼠心肌移植免疫耐受的可行性。方法通过逆转录病毒载体介导的基因转移技术,将供体小鼠(C57BL/6)的H-2Kb基因(小鼠MHC-类基因)导入到受体小鼠(BALB/c)的造血细胞。结果H-2Kb基因可以稳定地整合进小鼠造血细胞的基因内,并在受体内表达较长时间。正常对照小鼠的心肌移植物平均存活时间(MST)为9.4±1.84d。诱导耐受组小鼠的心肌移植物MST为45.7±6.001d,比对照组明显延长(P<0.05)。结论H-2Kb基因转移诱导了供体特异性的免疫耐受。

ObjectiveTo look for the effect of H 2K b gene transfer on myocardium transplantation tolerance in mice.Methods H 2K b gene of donor mouse(C 57 BL/6) was transferred into the recipient(BALB/c) murine hematopoietic cells by retrovirus mediated gene transfer technique.Results H 2K b gene could be transferred into murine hematopoietic cells successfully, and it can express stably long in the recipient mice. The median survival time(MST) of heart graft in the induced tolerance group was significantly longer than that of the control group(45.7±6.001 days vs 9.4±1.84 days, P <0.05).Conclusion Donor specific immune tolerance to allograft has been induced by H 2K b gene transfer. This may provide a novel way for inhibiting transplantation rejection in clinical transplantation. [