摘要
目的制备抗心肌肌钙蛋白T(cTnT)单克隆抗体,并用免疫组化方法诊断实验性缺血心肌部位、程度。方法常规免疫Balb/c小鼠,经细胞融合、克隆化,筛选抗cTnT杂交瘤细胞株,制备高效价、特异的单克隆抗体,采用ABC染色,分析缺血心肌部位及程度。结果在8株杂交瘤细胞株中选出IF9作为第一抗体。ABC染色后坏死区为粗颗粒棕褐色聚集着色,缺血区为小颗粒浅棕色着色,正常心肌不着色。结论应用抗cTnT单克隆抗体能特异诊断缺血心肌的部位、程度。
Objective To develope anti cardiac troponin T(cTnT) monoclonal antibody and identify region and degree of ischemic myocardium by immunohistochemistry.Methods Balb/c mice were immunized with cTnT.The specific,high title monoclonal anti cTnT antibody were developed after cell fusion,cloning and screening.The localization and degree of ischemic myocardium were analyzed by ABC stain.Results The IF 9 were selected as first antibody within eight hydridoma cell lines.After ABC stained,the area of necrosis stained bigger dense granular material,while ischemic myocardium remained uniformly small granular.Normal myocardial did not be stained.Conclusion The localization and degree of ischemic myocardium may be diagnosis by the specific monoclonal anti cTnT antibody. [
出处
《免疫学杂志》
CAS
CSCD
北大核心
2000年第1期63-65,共3页
Immunological Journal
基金
军队"九五"医药卫生科研青年基金! ( 970 3 10 5 5 )
广州市重点项目基金! ( 972 4 80 6)资助
关键词
心肌肌钙蛋白T
单克隆抗体
诊断
心肌缺血
cardiac troponin T
monoclonal antibody
ischemic myocardium
diagnosis
