温和酸消化法测定血清总碘含量

Detection of total iodine in serum by acid digesting under mild conditions

目的 测定血清中总碘含量。方法 温和酸消化法。结果 工作曲线在 0～ 2 5 0 μg/ L 范围内 ,r=- 0 .9985～ - 0 .9998,最低检测浓度为 15 .86 μg/ L(取血清 0 .2 ml)。标准及样品精密度变异系数均小于 10 % (n =6 )。当血清碘本底为 2 0 .7,32 .2 ,6 0 .2 μg/ L,加标量为 10 0 μg/ L 时 ,回收率分别为 98.47% ,97.40 % ,10 2 .5 7% (n=6 )。结论 利用温和酸消化法测定血清总碘含量 。

Objective To detect the total iodine in human serum.Methods Serum is digested by chloric acid under mild conditions.Results The coefficient of correlation of working curve are between - 0.9985 and -0.9998 when the iodine contents are between 0 and 250μg/L.Using 0.2ml of serum,the lowest test concentration is 15.86 μg/L.The variation coefficient of standards and samples are lower than 10%(n=6).The average rates of recovery are 98.47%,97.40%,102.57% when the serum iodine are 20.7,32.2,60.2μg/L,respectively,and added 100μg/L standard iodine.Conclusions Digesting the serum by acid under the mild conditions this method for detecting total iodine can get a good result. [