盐酸法提取污泥中蛋白质过程的研究

Study on the Processes of Protein Extraction from Sludge by Hydrochloric Acid

为了探索剩余污泥中蛋白质的提取方法,采用盐酸和氢氧化钠对污泥中蛋白质进行了提取,并对提取液进行了紫外光谱分析。结果证明盐酸提取法更有利于污泥中蛋白质的提取操作,并可以保证蛋白质质量。随后,实验考察了温度、污泥浓度、盐酸浓度以及操作时间对提取液中蛋白质浓度的影响情况。结果表明:蛋白质浓缩随温度呈先慢后快的增加趋势,随污泥浓度呈线性增加趋势,随盐酸浓度呈先快后慢增加趋势,适当延长时间有利于提升提取液中的蛋白质浓度。

In order to explore the methods of protein extraction from sludge,firstly,hydrochloric acid and sodium hydroxide were used to extract protein in this paper,and then the extracts were analyzed by UV spectrum.The results showed that hydrochloric acid extraction method was more conducive to the protein extraction and could ensure the protein quality.Subsequently,the effects of temperature,sludge concentration,hydrochloric acid concentration and operating time on protein concentration in the extract were experimentally investigated in detail.The results show that：the change trend of the protein concentration in the extract with temperature is slow after fast,with sludge concentration is linear,with hydrochloric acid is fast before slow,and appropriate extension of time will help to enhance the protein concentration in the extract.