两种激素干预对小鼠哮喘气道重塑模型的影响

Effects of Two Kinds of Coriaceous Hormone Theerapy on Airway Remodeling in Asthmatic Mice

目的 建立小鼠哮喘气道重塑模型,观察腹腔注射地塞米松和雾化吸入布地奈德对气道重塑的干预作用.方法 将32只BALB/C级小鼠随机分为4组：对照组（A组）、哮喘气道重塑模型组（B组）、地塞米松治疗组（C组）、布地奈德治疗组（D组）,每组8只.B,C,D组以卵蛋白致敏,后以卵蛋白反复激发,C组在每次激发前给予腹腔注射地塞米松,D组在每次激发前给予布地奈德雾化吸入,A组以生理盐水代替致敏和激发,解剖小鼠肺组织包埋、切片,行HE染色和Masson染色.然后分析HE染色下各组支气管壁总面积（Wat）和支气管壁平滑肌面积（Wam）,分析Masson染色下基底膜下胶原沉积面积（Wcol）.结果 B组小鼠支气管壁总面积（Wat）/基底膜周径（Pbm）、支气管平滑肌面积（Wam）/基底膜周径（Pbm）、胶原沉积面积（Wcol）/基底膜周径（Pbm）均高于A组正常对照组的小鼠（P〈0.05）;C组和D组的（Wat）/（Pbm）,（Wam）/（Pbm）,（Wcol）/（Pbm）都低于B组（P〈0.05）;C组和D组比较,无明显的差异（P〉0.05）.结论 小鼠哮喘形成过程中,气道重塑起着重要作用;腹腔注射地塞米松及雾化吸入布地奈德均能抑制气道重塑的形成,两者比较作用无差异.

Objective To establish the model of airway remodeling in asthmatic mice, and to investigate the effects of injected intraperitoneally by dexamethasone therapy and inhaled budesonide therapy on airway remodeling. Methods Thirty-two BALB/C mices were randomly divided into four groups ： control group （ A ）, airway remodeling model group （ B ）, dexamethasone therapy group （ C ）, and budesonide therapy group （ D ）. There 8 mices in each group. Each mice of group B, group C and group D treated with ovalbumin antigen （ OVA ） to sensitize asthma, and treated with ovalbumin antigen （OVA） to stimulate asthma repeatly. Each mice of group C was injected intraperitoneally by dexamethasone before stimulating. Each mice of group D inhaled Budesonide before stimlating. Each mice of group A used normal saline as a substitute for ovalbumin antigen （ OVA ） to sensitize and stimulate asthma. Lung tissues were sliced. Lung sections were stained with Hematox- , ylin-eosin（H＆E） stain and Masson＇s trichrome stain. The area of bronchial airway wall（Wat） and smooth muscle（Wam） were assessed by hematoxylin-eosin staining. The area of collagen deposition was assessed by Masson＇s trichrome staining. Results The areas of bronchial airway wall（Wat/Pbm） ,smooth muscle（Warn/Pbm） and collagen deposition（ Wcol/Pbm） of group B were higher than that of group A. Three areas of group C and group D were lower than that of group B. There was no differences between group C and group D. Conclusion Airway remodelling contributes to airway remodeling in asthmatic mice significantly. Injected intraperitoneally by dexamethasone therapy and inhaled budesonide therapy may function as an irihibitor of asthma airway remodelling. There is no diference in these two treatment method.