细胞外基质蛋白1对HaCaT细胞增殖及细胞周期的影响

Effect of ECMl on the proliferation and cell cycle of HaCaT cell

目的:确定细胞外基质蛋白1(ECM1)对HaCaT细胞增殖及细胞周期的影响。方法:将转染pEGFP-N2-ECM1的含有ECM1的MCF-7细胞上清液作用于HaCaT细胞。MTT法检测HaCaT细胞的增殖,FCM法测定细胞周期变化。结果:与对照组相比,经ECM1作用48h后,细胞G_1期比例显著降低,S期与G_2期比例则显著升高。结论:ECM1对培养的HaCaT细胞具有诱导增殖的作用。

Objective： To determine the effect of extraeellular matrix proteins - 1 （ECM1） on the proliferation and cell cycle of HaCaT cell. Methods： The supernatant containing ECM1 of pEGFP - N2 - ECM1 transfeeted MCF - 7 cell was added to HaCaT cells. MTr method was used to detect the proliferation of HaCaT cells and FCM method was used to measure the change of cellular cycle. Results： ECM1 showed positive effect on the proliferation after 48hs, when treated with different concentration. Compared with control group, the cells in G1 phase decreased, while cells in G2 and S phases increased. Conclusion： ECM1 can enhance the proliferation of HaCaT cell.