下边缘皮质中神经元周围基质网络(PNNs)的降解联合消退训练能够抑制吗啡奖赏记忆的复燃

DEGRADATION OF PERINEURONAL NETS IN INFRALIMBIC CORTEX COMBINED WITH EXTINCTION TRAINING INHIBITS REINSTATEMENT OF MORPHINE CPP

目的:利用硫酸软骨素酶(ChABC)降解下边缘皮质神经元周围基质网络(PNNs)之后,观察对于吗啡奖赏记忆复燃的影响。方法:采用条件性位置偏爱(CPP)模型,用核团微注射方法。结果:(1)与PBS对照组相比,消退训练前在下边缘皮质微注射ChABC将PNNs降解之后联合消退训练能够抑制消退后低剂量吗啡引起的CPP复燃;(2)与对照组相比,吗啡CPP训练前下边缘皮质微注射ChABC,对于吗啡CPP的获得没有影响。结论:本研究发现下边缘皮质神经元周围基质网络(PNNs)/硫酸软骨素蛋白聚糖(CSPGs)降解之后,可以抑制消退后低剂量药物引起的觅药行为的复现。这种作用可能是通过PNNs/CSPGs降解能够增强吗啡奖赏消退记忆来发挥作用的。

Objecti.v.e.：To study the role of perineuronal nets（PNNs）in infralimbie cortex subregion of medial prefrontal cortex（IL- mPFC）in extinction and reinstatement of morphine reward memory. Methods ： A conditioned place preference （CPP）procedure in rats and intracranial injections were used in this study. Results： We found that two groups of rats acquired morphine CPP after training, then the rats were injected with Chondroitinase ABC （ChABC） or vehicle into the infralimbic cortex. Compared with control group, ChABC injections combined with extinction training can prevent subsequent reinstatement of morphine CPP induced by a low dose of morphine. However, Intra -infralimbic cortex injection of ChABC had no effect on new learning of morphine CPP. Conclusion： Results indicate that infralimbic cortex PNNs degradation combined with extinction training can erase morphine reward memory. This finding supports the evidence that PNNs is a powerful modulator of inhibitory learning during extinction process.