我国棉花枯萎菌生理小种特异性DNA扩增片段的筛选及序列测定

SCREENING AND SEQUENCING OF RACE SPECIAL DNA FRAGMENTS OF Fusarium oxysporum f.sp. vasinfectum IN CHINA

利用 RAPD技术 ,以随机引物对我国棉花枯萎菌的 3个生理小种 (3、7、8号小种 )共 2 6个菌株进行 PCR扩增 ,从产生的 140个 RAPD分子标记中寻找到了不同小种的特征性条带 ,OPF- 10 513 (3号小种 )、OPF- 0 83 71(7号小种 )及 OPF- 12 70 3 (8号小种 )。将其纯化后克隆到 p GEM- T Easy质粒载体上 ,并获得了 DNA特异性片段的核酸序列。

Random amplified polymorphic DNA(RAPD) technique was used to PCR amplification of 26 strains of races 3,7 and 8 of Fusarium oxysporum f.sp. vasinfectum . The race special DNA fragments OPF 10 513 (race 3),OPF 08 371 (race 7) and OPF 12 703 (race 8) were obtained from 140 RAPD markers, which have been cloned to pGEM T Easy plasmid, and sequences of those DNA fragments have also been achieved.