大黄蟅虫丸抗四氯化碳致大鼠肝纤维化的实验研究

Protective Effects of Dahuangzhechongwan against Rat Hepatic Fibrosis Induced by Carbon Tetrachloride

目的探讨大黄蟅虫丸抗肝纤维化的作用机理。方法建立四氯化碳(CCl4)复合因素肝纤维化大鼠模型,观察大黄蟅虫丸对模型动物转化生长因子β1(TGFβ1)、基质金属蛋白酶组织抑制因子1(TIMP-1)的影响。结果大黄蟅虫丸能显著抑制大鼠血清中的TGFβ1、TIMP-1、透明质酸酶(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)、IV型胶原(CIV)、谷丙转氨酶(ALT)、谷草转氨酶(AST)的表达。结论大黄蟅虫丸对肝纤维化形成中两个重要因子TGFβ1和TIMP-1表达的抑制作用,可能是其抗肝纤维化的作用机理之一。

ObjectiveTo explore the protective mechanism of Dahuangzhechongwan on hepatic fibrosis. MethodsRat fibrosis model was established by CCl4 compound factors.Transforming growth factor β1（TGFβ1）,tissue inhibitor of metalloproteinase-1（TIMP-1） were detected in the serum among different groups. ResultsDahuangzhechongwan could reduce the serum levels of TGFβ1,TIMP-1,Hyaluronic Acid（HA）,Laminin（LN）,Procolagen Type Ⅲ（PCⅢ）,Collagen TypeⅣ（CⅣ）,glutamic-pyruvic transaminase（ALT） and glutamic-oxalacetic transaminase（AST）,respectively. ConclusionDahuangzhechongwan can inhibit the expression of TGFβ1 and TIMP-1,the two main factors were related to the occurrence of hepatic fibrosis.This may be one of the mechanisms which Dahuangzhechongwan restrains the progression of liver fibrosis.