摘要
目的测定深绿卷柏中穗花杉双黄酮和橡胶树双黄酮的含量。方法采用梯度洗脱的反相高效液相色谱法,选用Agilent ZORBAX-SB C18(5μm,4.6 mm×250 mm)色谱柱,以乙腈-0.1%冰醋酸为流动相,在25℃柱温,检测波长为254nm时检测,建立了深绿卷柏药材的质量评价标准。结果穗花杉双黄酮、橡胶树双黄酮分别在0.33～3.3μg/ml和0.27～2.7μg/ml浓度范围内与峰面积呈良好的线性关系,相关系数分别为r=0.993和r=0.990;回收率分别为:95.36%(n=6,CV=1.79%)和95.49%(n=6,CV=2.61%)。结论 RP-HPLC法同时测定深绿卷柏中穗花杉双黄酮和橡胶树双黄酮的含量,方法灵敏、简便、准确;可用于评价深绿卷柏药材的品质。
ObjectiveTo determine the contents of amentoflavone and heveaflavone in Selaginella.doederleinii Hieron. Methods The method of RP-HPLC was adopted with gradient elusion.The chromato-graphic volumn was Agilent ZORBAX-SB C18（5μm,4.6 mm× 250 mm）,the mobile phase was acetonitrile-0.1% acetric acid solution,the detection wavelength was at 254nm and the column temperature was 25℃. ResultsAmentoflavone and heveaflavone showed good linear relationship within the range of 0.33～3.3（μg/ml）,r=0.993 and 0.27～2.7（μg/ml）,r=0.990,respectively.The recovery rate was 95.36%（n=6,CV =1.79%） and 95.49%（n=6,CV=2.61%）,respectively. ConclusionThe method of RP-HPLC is sensitwe,simple,accurate and can be used to evaluate the quantity of Selaginella doederleinii Hieron.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2012年第4期854-857,共4页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.21005089)
