克氏原螯虾雄性生殖系统的超微结构

Histological studies on the male reproductive system of Procambarus clarkii

采用透射电镜研究了克氏原螯虾输精管的超微结构,采用石蜡切片法研究了克氏原螯虾的精巢、输精管和精荚的显微结构,并对精巢做了周年变化研究。结果表明,精巢由许多生精小囊和收集管组成,其结构呈葡萄串状,生殖细胞在生精小囊内发育成熟后由收集管输送到输精管;输精管有左右两条,相对于左侧输精管,右侧输精管比其约长一半,直径更大,卷曲程度更高,内容物含量更多,分泌细胞内的内质网含量更丰富;输精管根据形态不同分为前、中、后、生殖突4段,前中段具有分泌精荚壁物质的功能,后段具有储存、射出精荚的功能,末端生殖突为输精管出口;精荚呈长囊形或椭球形,由精子团、精荚基质和两层精荚壁组成,精子团偏向精荚一侧分布;克氏原螯虾雄虾精巢在一周年中的5—8月和10月处于精子细胞期的生精小囊比例很高,具有两个成熟时期。

There are scarce researches about the male reproductive system of Procambarus clarkii.This study aims to get some features of the male reproductive system of P.clarkii.The ultrastructure of the vas deferens was studied by using transmission electron microscopy,the microstructure of testis,vas deferens and spermatophore of P.clarkii was studied by using paraffin method,and the annual variation of the testis was investigated.Testis comprised many spermatogenic acini and collecting ducts whose structure is like grapes;The germ cells in the spermatogenic acini are transported to the vas deferens by through the collecting duct after matured;P.clarkii has a right and a left vas deferens,the right vas deferens was half longer than the left,the right vas deferens has a larger diameter,its curling degree and inclusion content are materially higher than the left,and secretory cells of the right vas deferens have more abundant endoplasmic reticulum.The vas deferens can divide into anterior,middle,posterior sections and a male gonopores acording to the difference of its morphous.Anterior and middle vas deferens can secrete materials for the formation of the spermatophore.The posterior section can store and ejaculate spermatophore.Male gonopores are the outcome of the vas deferens.The shape of spermatophore is elongated capsule or ellipsoidal.The spermatophore is composed of sperm mass,spermatophore matrix and two layers of spermatophore wall,sperm mass is in one side of the spermatophore.Male P.clarkii has two maturity periods that the most part of spermatogenic acini in the testis are at spermatid stage in its annual variation,and one is from May to August and the other is in October.