IL-17及IL-22在大鼠肝移植排斥反应中的表达及意义

Expression and significance of IL-17 and IL-22 in rat liver transplant rejection and tolerance

目的探讨IL-17及IL-22在大鼠肝移植排斥模型及耐受模型中的表达差异及意义。方法建立大鼠原位肝移植动物模型,分为排斥组和耐受组(各15只),取术后1、3、5、7 d大鼠血浆及肝组织标本,应用ELISA检测其血浆IL-17浓度,应用免疫组化染色、Western blot及荧光定量PCR检测其肝组织内IL-17及IL-22的细胞、蛋白及mRNA的表达情况。结果大鼠原位肝移植模型建立成功,结果显示排斥组IL-17血浆浓度显著高于耐受组;在肝组织中,IL-17及IL-22的淋巴细胞阳性率、蛋白表达水平及mRNA表达水平排斥组显著高于耐受组,且排斥组肝组织中STAT3 mRNA表达水平升高。结论 IL-17及IL-22参与肝移植术后急性排斥反应的发生过程,并推测其通过激活STAT3通路发挥效应。

To investigate the expression and significance of IL-17 and IL-22 in rat orthotopic liver transplant rejection and tolerance,we established the rejection and tolerance models in rats（n = 15）.The concentrations of IL-17 in plasma were measured by ELISA.And immunohistochemistry,Western blot,and real-time quantitative reverse transcriptase polymerase chain reaction（RT-PCR） were used to detect the IL-17 and IL-22 expression of cell level,protein level and mRNA level in liver.On 1,3,5,and 7 days post-transplantation,the concentrations of IL-17 in plasma of rejection group were higher than that of tolerance group.In rejection group,the expression levels of IL-17 and IL-22,including the frequencies of positive lymphocytes,protein level,and mRNA level,were significantly increased,as compared to tolerance group.Similarly,the expression of STAT3 mRNA in liver of rejection group was higher than that of tolerance group.The results suggested that IL-17 and IL-22 are participated in the development of graft rejection after liver transplantation,and worked via STAT3 activation.