摘要
目的观察MUC1的模拟表位肽对表达人MUC1 T739荷瘤小鼠的治疗作用。方法建立稳定表达人MUC1 T739荷瘤小鼠,培养T739小鼠成熟树突状细胞,荷瘤小鼠随机分为4组,用负载模拟表位肽成熟DC免疫表达人MUC1 T739荷瘤小鼠,第1组皮下注射等渗盐水,第2组注射空DC,第3组注射负载1号肽的DC,第4组注射负载2号肽的DC,测量肿瘤大小,计算肿瘤体积,称瘤重,并观察小鼠存活期。结果两组DC+肽免疫的小鼠瘤体积减小明显,与1、2组相比差异非常显著(P<0.01)。平均瘤重1、2组与3、4组相比差异非常显著(P<0.01)。第1～4组荷瘤鼠的生存期分别为(34.6±3.507)d,(35.2±3.564)d,(58.4±5.595)d,(59±6.819)d,3、4两组荷瘤小鼠生存期较1、2组明显延长,差异有显著性意义(P<0.01)。结论 MUC1模拟表位肽能明显抑制表达人MUC1 T739荷瘤小鼠肿瘤生长,显著延长T739荷瘤小鼠生存时间。
This study designed to investigate the functional immunological responses and effectiveness in MUC1-positive bladder cancer treated by the mimic peptide epitopes.T739 mice were subcutaneously inoculated with BST739-PC cells or BST739-MUC1 cells,then dendritic cells（DCs） derived from T739 tumor-bearing bone marrow were cultured.Experimental mice were randomly divided into 4 groups,which were subcutaneously injected with BST739-MUC1 cells.After 7 d,4 group mice were subcutaneously injected with PBS,unloaded DCs,number 1 peptide-loaded DCs and number 2 peptide-loaded DCs,respectively,and followed by the second injection on 14 d.Neoplastic diameters and neoplastic weight were measured and mouse survival was also monitored.The volume and the weight of tumor in two later groups was cut down and significantly differed from two former groups（P 0.01）.The survival of mice in PBS,unloaded DC,DC loaded with number 1 peptide and DC loaded with number 2 peptide group was（34.6±3.507） d,（35.2±3.564） d,（58.4±5.595） d,（59±6.819） d,respectively.The survival advantage of the groups receiving DC/No1 peptide or DC/No2 peptide treatment over the control groups receiving DC/PBS or PBS is statistically（P 0.01）.The growth of BST739-MUC1 tumor was depressed and the life duration of the BST739-MUC1-bearing mice treated with mimic epitope peptide-pulsed DC was prolonged significantly.All this result indicated that MUC1 mimic peptide epitopes cold be an important tumor-vaccine candidate for bladder cancer therapy.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2012年第5期394-397,402,共5页
Immunological Journal
基金
国家"863"计划资助项目(2002AA214111)
