蜜蜂消化道酶谱带分布及其糖转化酶活性

Band distribution of digestive enzymes in alimentary canal of bee and its α amylase activities

采用聚丙烯酰胺凝胶电泳分离意蜂和中蜂消化道各部分 (前肠、中肠、后肠 )肠液 .结果表明意蜂和中蜂消化酶的种类有明显差别 .它们的前肠均中有 1条酶带 (R1带 ) ,中肠均有 5条酶带 (意蜂为 R1、R3 、R4 、R5、R9;中蜂为 R1、R3 、R4 、R6、R10 ) ,意蜂后肠有 5条酶带 (R1、R2 、R4 、R7、R8) ,中蜂则没有 .意蜂和中蜂的前肠、中肠、后肠的糖化酶活性均依次降低 .前肠和中肠的糖化酶活性意蜂较中蜂低 。

Extracted solutions of different parts (foregut, midgut and hindgut) of alimentary canal of bee were isolated by polyacrylamide gel electrophoresis. The results showed that there was significant difference of the sorts of digestive enzymes between Apis mellifera ligustica and Apis cerana cerana. In details there was only one band (R 1) in foregut of both A.m.ligustica and A.c.cerana, while five bands in their midgut (R 1, R 3, R 4, R 5, R 9 in A.m.ligustica and R 1, R 3, R 4, R 6, R 10 in A.c.cerana), but there were five bands in hindgut of A.m.ligustica and none in A.c.cerana. Meanwhile, α amylase activities in foregut, midgut and hindgut of both bees were decreased in order and higher in foregut and midgut of A.c.cerana than those of A.m.ligustica while no significant difference in hindgut of both bees.