双歧杆菌对应激模型大鼠肠道通透性的影响

Influence of Bifidobacterium on the Intestinal Permeability in Stress Model Rats

目的:研究双歧杆菌对应激模型大鼠肠道通透性的影响及其与蒙脱石散联用的协同作用。方法:取SD大鼠50只随机均分为正常对照组、模型对照组、双歧杆菌干预组(2×108cfu)、蒙脱石散干预组(0.6 g.kg-1)及其联合干预组(双歧杆菌2×108cfu+蒙脱石散0.6 g.kg-1),每日灌胃给药1次,连续给药7 d,后4组每日给药后以避水压力模型(WAS)方法建立大鼠应激模型。第8天各组分别灌胃给予探针药物甘露醇80 mg、三氯蔗糖60 mg,检测各组大鼠给药后5、24 h尿液中甘露醇、三氯蔗糖的量及其比值,以评价肠道通透性。实验结束处死大鼠,检测各组大鼠血清中促肾上腺皮质激素释放因子(CRF)和促肾上腺皮质激素(ACTH)的含量。结果:与正常对照组比较,模型对照组大鼠24 h尿液中甘露醇的量及血清中CRF、ACTH含量均明显升高(P<0.05);与模型对照组比较,双歧杆菌干预组和联合干预组ACTH含量均明显降低(P<0.05),其余指标均有降低,但没有统计学意义。结论:双歧杆菌对应激模型大鼠的肠道通透性有影响,其与蒙脱石散联用对应激所致的肠道屏障功能紊乱无协同作用。

OBJECTIVE： To study the improvement effect of Bifidobacterium on intestinal permeability in stress model rats,and synergistic reaction of Bifidobacterium combined with Montmorillonite powders.METHODS： 50 SD rats were randomly divided into normal control group,model control group,Bifidobacterium treatment group（2×108 cfu）,Montmorillonite powders treatment group（0.6 g·kg-1） and drug combination group（Bifidobacterium 2×108 cfu＋Montmorillonite powders 0.6 g·kg-1）.Those rats were given medicine once a day via i.g.for consecutive 7 days.Stress model was established by WAS in the latter 4 groups.On 8th day each group was given mannitol 80 mg and sucralose 60 mg,and the amount and ratio of mannitol and sucralose in urine were determined 5 h and 24 h after administration to evaluate intestinal permeability.Rats were sacrificed at the end of experiment,and the content of corticotrophin releasing factor（CRF） and adrenocorticotrophic hormone（ACTH） in serum were determined.RESULTS： Compared with normal control group,the 24 h mannitol concentration,the serum levels of CRF and ACTH in model control group increased significantly（P0.05）.Compared with model control group,serum levels of ACTH in Bifidobacterium treatment group and drug combination group decreased significantly（P0.05）.Other parameters all decreased but had no statistical significance.CONCLUSION： Bifidobacterium can improve intestinal permeability of stress model rats,protect intestinal mucosa.In the treatment of intestinal dysfunction caused by chronic psychological stress,Bifidobacterium and Montmorillonite powders are not in synergies.