摘要
Recombinant human-salmon calcxtonin （hsCT） was synthesized and expressea on the cell surtaces ot recombinant Saccharomyces cerevisiae （yAGA2-hsCT） using the pAGA2-hsCT expression vector. Expression of recombinant hsCT was verified by flow cytometer.The resorption activity of osteoclasts was inhibited by recombinant hsCT protein which released from the cell surface of yAGA2-hsCT. Blood calcium determination in rats showed the activity of yAGA2-hsCT following oral administration of 5 mg/kg lyophilized transformant yeast to hypercalcemia rats decreases the serum calcium from 2.82 ±0.023 mM to 2.662 ± 0.012 mM.
