一例梅花鹿致病菌的分离、鉴定及药敏试验

Isolation,Identification and Drug Sensitive Test of Pathogenic Bacterium from a Spotted Deer

本研究主要是从江西省新余市某梅花鹿养殖场送检来的一只病死梅花鹿崽中分离细菌,进行培养特性观察、生化鉴定、动物试验和体外药敏试验,从而为临床诊断治疗提供理论指导。试验结果表明,从病死梅花鹿的心脏、肝脏、肺脏各分离到一株革兰氏阴性,短杆状细菌;通过培养特性观察和生化鉴定确定该病由大肠杆菌引起;通过试验动物攻毒试验得出3株菌都是强毒力致病菌,为本病例的病原菌;对于3株菌在体外进行药物敏感性试验,得出3株菌对药物的耐药性都非常强,而且敏感性都或多或少存在差异,对于3株菌都较敏感的药物为痢特灵、卡那霉素、庆大霉素;不敏感的药物有先锋Ⅴ、先锋Ⅵ、阿莫西林、左氧沙星。

The main purpose of this study was the isolation of pathogenic bacterium from a died spotted deer from a Xinyu farm in Jiangxi province,bacterial isolating culture,biochemical identification,animal experiments and drug sensitivity text in vitro were performed,and further guided clinical diagnosis and treatment.The results showed that,three strains pathogenic bacterium were isolated from the liver,heart and lung of the died spotted deer,they were all gram-negative short bacillus.By bacterial isolating culture and biochemical identification which indicated that the disease of the spotted deer was caused by Escherichia coil.The three strains were virulent pathogens,the conclusion was showed by the animal experiments.The results of drug sensitivity test in vitro showed the three strains were strong drug-resistant,and the sensibility of three strains was different.The three strains were sensitive to furazolidone,kanamycin,gentamicin ceftriaxone,but resistant to cefazdin,cefradine,amoxicilin,levofloxach.