融合蛋白d-EGF的结构预测及其意义

Significance of Prediction to of Fusion Protein d-EGF

目的对融合蛋白d-EGF二级、三级结构进行预测，为构建相应结构重组分子的可能性提供理论指导。方法以融合蛋白基因序列为基础。用DNAStat软件预测其蛋白质的二级结构；用InsightII软件对其三级结构进行建模预测。结果DNAStar软件的GamierRobson方法预测融合蛋白d-EGF含较多争折叠结构；ChargeDensity-Charge显示其3～22，31～48区段含丰富的正电荷。Insight II软件对其三级结构进行建模显示融合蛋白d-EGF存在一个由六个链内二硫键组成曲结构域，同时存在3个反向平行的β-折叠结构，这些结构是融合前β-defensin-3，EGF两分子生物学活性所必需的部分。结论融合蛋白d-EGF的二、三级结构预测结果显示该组合分子可能具有β-defensin-3，EGF两种分子的功能，该方法可以为构建具有相应结构重组蛋白的可能性提供理论基础。

Objective The secondary structure and tertiary structure of d-EGF were predicted by DNAStar and Insight II software,which provides theory direction for probability that constructs recombinant molecules with special function. Methods The secondary structure of fusion protein d-EGF was predicted by the software of DNAStar. Tertiary structure of target protein was predicted by the Insight II software. Results Recombinant protein d-EGF contained more E-sheet regions by Gamier Robson method of DNAStar and have more positive charge in 3-22,31 -48 areas by Charge Density-Charge method of DNAStaf, The six-chain disulfide bond and three anti-parallel β-pleated sheet structures lie in the recombinant molecule. These are necessary to their activities in β-defensin-3, EGF molecules which reformed d-EGF. Conclusion Secondary and tertiary structure of d-EGF could ba predicted successfully and its preliminaryactivities could be analyzed by software,which would be the theory foundation of the special recombinant molecules.