富血小板血浆血小板浓度与全血血小板浓度和红细胞比容相关性探讨

Study on Relativity of Platelet Count of Platelet-rich Plasma and Platelet Count,Hematocrit of Whole Blood

目的探讨富血小板血浆（PRP）血小板浓度与全血血小板浓度、红细胞比容（HCT）相关性。方法随机收集162例门诊体检志愿者静脉血标本，以EDTA-K2，枸橼酸钠抗凝。枸橼酸钠抗凝血800r／min（离心半径19cm）离心5min，分离富含血小板血浆（PRP），应用SysmexXE-2100血液分析仪测定全血血小板浓度（X-）和HCT（X2），PRP血小板浓度（Y）。以HCT0．35为界，将数据分为正常组和低值组。结果所得数据采用多元相关性统计分析得到回归方程Y总=1.30951X1＋744．2945X2—262．068（R2=0．8978）；Y正常组=1．380208X1＋855．8848X2—323．374（R=0．8929）；Y低值组=1．088972X1＋465．2288X2—123．101（R2=0．9611）。结论全血血小板浓度、红细胞比容与富血小板血浆血小板浓度相关显著，由全血血小板浓度和红细胞比容可初步推算富血小板血浆血小板浓度。

Objective To explore relativity of platelet count of platelet-rich plasma, platelet count and hematocrit of whole blood. Methods Venous blood sample of outpatient volunteers were collected and anticoagulaged by EDTA-K2 and sodium citrate（ n= 162） ,and platelet-rich plasma（PRP） was obtained by centrifuging the blood anticoagulaged by sodium citrate at a low speed （800 r/min, r= 19 cm） for 5 minute, then platelet number of PRP（Y） ,platelet number （X1） and hematoerit （X2） of whole blood were detected by Sysmex XE-2100 blood analyzer. The data was divided into normal and low group by 0. 35 of hematocrit. Resells Regression equation analysed by multiple linear regression： Ytotal = 1. 309 51 X1 ＋ 744. 294 5X2- 262. 068（R2 =0. 897 8） ;Ynonnal=1. 380 208X1＋855. 884 8X2-323. 374（R2 =0. 892 9） ;Y low =1. 088 972X1＋465. 228 8X2-123. 101 （R2 = 0. 961 1）. Conclusion Platelet count of the platelet-rich plasma,platelet count and hematoerit of the whole blood seem to relate significantly, and platelet count of platelet-rich plasma could be estimated approximately by platelet count and hematocrit of the whole blood.