沙漠地区自然环境中消毒包储存时限及细菌来源研究

Study on storage time and bacterial origin of disinfection package under natural environment in desert region

[目的]探讨沙漠地区自然环境中消毒包有效储存时限以及消毒包内生长细菌的来源。[方法]双层棉布手术敷料包、器械包、敷料器械混合包各54个,预真空压力蒸汽灭菌后分别存放在医疗战备仓库自然环境的密闭储存箱(密闭组)和开放储存箱(开放组)内,每个箱内放置3种类型消毒包各3个,于灭菌后第14天、第24天、第34天、第44天、第54天、第64天、第74天、第84天、第94天取样,每次每组随机取9个包进行细菌培养,同时对仓库空气、储存箱取样培养;根据消毒包内细菌种类筛选空气中同种细菌不同菌株,提取基因组DNA,以其为模板,对16SrRNA和23SrRNA的转录间隔区(ITS)序列进行PCR扩增,扩增产物回收测序,将测得的基因序列用Blast进行比对。[结果]灭菌后84d两组消毒包组细菌培养阴性,94d两组消毒包细菌培养阳性;消毒包生长细菌与空气中同种异株菌16SrRNA～23SrRNA ITS序列相似性为98%～100%。[结论]沙漠地区自然环境相对洁净、干燥的空气,有利于延长消毒包的有效储存时限;消毒包生长的细菌来源于空气。

Objective：To probe into the effective storage time of disinfection package in the natural environment in desert area,as well as bacterial growth source in disinfection package.Methods：54 double cotton surgical dressings packets,54 equipment packets and 54 mix dressing equipment packages were stored in the sealed storage boxes and open storage boxes in medical readiness warehouse under natural environment.After vacuum pressure steam sterilization,3 bags in each types of disinfection package were deposited in each box,then the sample was taken respectively on 14th days,24th days,34th days,44th days,54th days,64th days,74th days,84th days,94th days after sterilization,9 packets were taken for bacterial culture in each group in each time,at the same time,the air and storage tanks in warehouses were taken randomly for sampling culture;the different strains of the same species of bacteria were screened in air according to bacterial species in disinfection package,and they were extracted genomic DNA,which was taken as the template,16S rRNA and 23S rRNA transcribed spacer（ITS）sequence for PCR amplification,PCR amplification products were recovered,sequenced,the got gene sequence were compared with Blast.Results：On the 84d after sterilization,bacterial culture of disinfection package in two groups was negative,on the 94d the bacterial culture was positive;ITS sequence of bacteria growing in disinfection package was similar to the air allogenic strains 16S rRNA-23S rRNA,and the similarity reached 98%-100%.Conclusion：The relatively clean and dry air under the natural environment in desert area is profit for prolonging the effective storage time limit of disinfection package;growth of bacteria in disinfection bag is from the air.