染色体畸变率估算32P的辐射剂量

The quantitative radiation dose of 32p by chromosomal aberration

目的应用G冠带的染色体双着丝粒畸变率评估32P的辐射剂量。方法应用4MV的X射线对离体人血液进行0．5Gy、1Cy、2Gy和4Gy的照射，建立染色体双着丝粒畸变率和x射线的辐射剂量的剂量-效应曲线。将74kBq的32P胶体加入淋巴细胞的培养液后72h，进行G显带染色体分析，通过x射线的辐射剂量和染色体双着丝粒畸变率的剂量-效应曲线来评估32P的辐射剂量。结果4MV的x射线的辐射剂量和染色体的双着丝粒畸变率呈线性正相关，剂量-效应曲线为y=24．05x-13．34（R2=0．975）。74kBq的32P胶体产生的染色体双着丝粒畸变率为18％，即74kBq的32P胶体在5ml淋巴细胞培养液中72h约产生1．3Gy的辐射剂量。结论应用染色体畸变率可以有效地评估放射性核素的内照射剂量.

Objective To quantify the radiation dose of 32p by the G-banding chromosomal aberration rate. Methods The human blood was irradiated by 4 MV X-ray of 0.5, 1, 2, 4 Gy respectively. The doseresponse curve between the radiation dose and the dicentrics aberration rate was assayed. 74 kBq 32p colloid was put into culture solution and, after 72 h, the dicentrics aberration rate was observed. The radiation dose of 32p was assayed by the dose-response curve. Results The dose-response curve between the radiation dose and the dicentrics aberration rate was y=24.05x-13.34 （R2=0.975）. The dicentrics aberration rate of 74 kBq 32p was 18% and the radiation dose of 32p in 5 ml culture solution during 72 h was 1.3 Gy. Conclusion The radiation dose of radionuclide can be estimated by chromosomal aberration rate.