益肾调周法对卵巢早衰模型大鼠卵巢储备能力的影响

Effect of Yishen tune-periods method to ovarian reserve capacity in POF model rats

目的观察益肾调周法对卵巢早衰大鼠的卵巢储备能力的影响,探讨其量效关系及作用机制。方法将大鼠模型随机分为空白组(A)、模型组(B)、克龄蒙组(C)、滋肾填精汤高(D1)、汤低剂量组(D2)、温肾填精汤高剂量组(E1)、温肾填精汤低剂量组(E2)、益肾调周法高剂量组(F1)、法低剂量组(F2),A,B组给予每日灌胃两次蒸馏水,各给药组每日灌胃1次。连续用药20 d。于实验末次给药后,各组大鼠断头采血,取血清测定血清中E2值、FSH水平;检测AMH,INHB。结果 (1)各个治疗组的血清E2水平均较模型组有提高,血清FSH的水平均有降低;(2)与模型组比较,各组的AMH水平均有提高,INHB的表达均增强,组间对比差异有统计学意义。结论益肾调周法可以宏观的调整血清中FSH、LH、E2、AMH、INHB的含量,增强卵巢的敏感性并改善卵巢的储备能力,其高剂量组的疗效显著。

Objective Observation the effect of Yishen tune-periods method to ovarian reserve capacity in POF model rats in order to discusse dose-effect relationship and interaction mechanism,it can provide the scientific basis in prevention.Methods The rats were randomly divided into blank group(A),the rest of animal subjects are given by the injection of cyclophosphamide to establish POF model.POF model rats were randomly divided into namely model group(B),climen group(C),zishentianjing soup highdose group(D1),zishentianjing soup lowdose group(D2),wenshentianjing soup highdose group(E1),wenshentianjing soup lowdose group(E2),yishen tune-periods method highdose group(F1),yishen tune-periods method lowdose group(F2).A,B group were poured distilled water into stomach twices daily,each group was poured once daily.consecutive medication last 20 days.After the last medication,all rats were taken blood in decollation for blood serum.detect the serum levelof E2、FSH;detect the serum levelof AMH,INHB.Result ①each treatment group of serum level of E2 were increased compared with model group;Each treatment group of serum level of FSH were decreased;②each treatment group of serum level of AMH were increased compared with model group,each treatment group of INHB expression were enhanced compared with model group.Among groups had significant difference;Conclusion Yishen tune-periods method can adjustment macro serum level of FSH,LH,E2,AMH,INHB and strengthen to improve ovarian reserve capacity,the high dose group of curative effect is distinct.