火针对类风湿性关节炎大鼠血清白介素-1和肿瘤坏死因子-α的影响

Effect of Fire-Needle Intervention on Serum IL-1 and TNF-α Levels of Rheumatoid Arthritis Rats

目的:观察火针对类风湿性关节炎大鼠血清白介素-1(IL-1)和肿瘤坏死因子-α(TNF-α)的影响,探讨火针治疗类风湿性关节炎的作用机制。方法:将40只雌性Wistar大鼠随机分为正常组、模型组、甲氨蝶呤(MTX)组、火针组。于大鼠右后足跖底部皮内注射0.1mL完全弗氏佐剂建立类风湿性关节炎模型。火针点刺"夹脊穴""足三里"和"阿是穴",共治疗8次。容积法测量大鼠右后足肿胀度;双抗体夹心ABC-ELISA法检测大鼠血清IL-1和TNF-α含量;光镜观察大鼠膝关节滑膜组织的病理变化。结果:模型组大鼠足部肿胀度、血清IL-1和TNF-α的含量较正常组显著升高(P<0.01);MTX组造模后33d足部肿胀度较模型组降低(P<0.01),火针组造模后19、26、33d足部肿胀度较模型组降低(P<0.01),治疗后MTX组和火针组大鼠血清IL-1和TNF-α的含量较模型组均明显降低(P<0.05,P<0.01),火针组与MTX组相比大鼠足部肿胀度、血清IL-1和TNF-α的含量明显降低(P<0.05,P<0.01)。与正常组比较,模型组大鼠滑膜组织有明显的病理改变,各治疗组均能改善膝关节滑膜组织的炎性病变,且火针对滑膜病变的改善优于MTX。结论:火针可降低类风湿性关节炎大鼠血清IL-1和TNF-α的含量,减轻关节局部肿胀度,改善滑膜病变,有效控制类风湿性关节炎的病情发展。

Objective To observe the effect of fire-needle intervention on serum IL-1 and TNF-α contents in rheumatoid arthritis（RA） rats so as to study its underlying mechanism in improving RA.Methods A total of 40 Wistar rats were randomized into normal control,model,methotrexate（MTX,medication） group and fire-needle groups,with 10 cases in each group.RA model was established by injecting Freund＇s complete adjuvant（0.1 mL） into the rats＇ right hind paw.Fire-needle was applied to＂Jiaji＂（EX-B 2）,＂Zusanli＂（ST 36） and ＂Ashi point＂（the most severe site of the swelling joint）,once every three days and 8 times altogether.The rat＇s right hindpaw swelling volume was detected by volumetric method,and serum IL-1 and TNF-α contents were determined by double antibody sandwich ELISA.The pathological changes of synovium tissue of the right knee-joint were observed by optical microscope.Results In comparison with the control group,the swollen paw volumes at time-points of 12 d,19 d,26 d and 33 d after modeling and serum IL-1 and TNF-α contents were increased significantly（P0.01）.Compared to the model group,the swollen paw volumes on day 33 in both medication and fire-needle groups were decreased considerably（P0.01）,while serum IL-1 and TNF-α contents in the medication and fire-needle groups were reduced significantly（P0.05,P0.01）.No significant differences were found between the medication and fire-needle groups in the swollen paw volumes on day 12（P0.05）.The pathological states（synovial cells＇ arrangement,inflammatory infiltration,synovial membrane thicke-ning,etc.） of synovium tissue of the right knee-joint in both medication and fire-needle groups were relatively lighter than those of the model group.Conclusion Fire-needle therapy can down-regulate serum IL-1 and TNF-α contents in rheumatoid arthritis rats,and reduce the degree of arthroncus of the affected joint,which may contribute to its effect in improving synovial lesions.