15-脱氧前列腺素J_2对非酒精性脂肪肝大鼠影响的研究

Effects of 15-Deoxidizing Prostaglandin J_2 to rats with nonalcoholic fatty liver disease

目的研究15-脱氧前列腺素J2(15d-PGJ2)对非酒精性脂肪肝(NAFLD)大鼠的治疗作用。方法高脂饲料喂养Wistar大鼠12周制备NAFLD大鼠模型,再将其随机分为HI组(15d-PGJ2高剂量干预组)、LI组(15d-PGJ2低剂量干预组)、MC组(模型对照组);将普通饲料喂养大鼠作为NC组(正常对照组)。干预2周后检测肝组织匀浆中三酰甘油(TG)、总胆固醇(TC),血清丙氨酸转氨酶(ALT)、TG、TC及血糖(GLU)的含量,观察各组大鼠的肝组织病理学变化。结果高脂饮食可引起大鼠肝脏脂肪变性。HI组所有检测指标及LI组肝内TC水平均高于NC组;LI组和NC组所有检测指标及HI组ALT、TC、TG水平均低于MC组;HI组ALT、TG、GLU水平均高于LI组,差异有统计学意义(P<0.05)。HI组、LI组肝组织脂肪变性程度较MC组改善更明显。结论 15d-PGJ2可有效改善NAFLD大鼠的肝脏病变。

Objective To study the therapeutic action of 15-Deoxy-△12,14-prostaglandin J2（15d-PGJ2） on nonalcoholic fatty liver disease（NAFLD）in rats.Methods Wistar rats were fed with high-lipid diet for 12 weeks to induce nonalcoholic fatty liver model.Then randomly divided into 15d-PGJ2 higher dose intervention group（HI）,15d-PGJ2 lower dose intervention group（LI）,and model control group（MC）.Ordinary diet fed rats were regarded as group NC（normal controls）.After two weeks intervention,observe the liver tissue pathology change.The triglyceride（TG）,total cholesterol（TC）of liver and alanine aminotransferase（ALT）,TG,TC,glucose（GLU） of blood were measured by biochemisty methods.Results All indexes of intrahepatic of group HI and TC of group LI were higher than that in the NC group;all indexes of LI group and NC group and ALT,TC,TG in HI group lower than MC group;ALT,TG,GLU in group HI were higher than those in LI group,the difference was statistically significant（P0.05）.Liver steatosis degree was improved more obviously In group HI and group LI,when compared with MC group.Conclusion The 15d-PGJ2 can effectively improved the liver lesions of the rats with NAFLD.