抗戊唑醇禾谷镰孢菌的相对渗透率变化

Changes of Relative Leakage of Fusarium graminearum Resistant to Tebuconazole

通过比较敏、抗菌株菌丝体在不同浓度戊唑醇溶液中的相对渗透率动态变化,研究了禾谷镰孢菌Fusarium graminearum对戊唑醇的抗药性与相对渗透率的关系。结果表明:在戊唑醇溶液浓度为1μg/mL和0.0451μg/mL(EC50)时,敏感菌株的相对渗透率变化明显大于抗性菌株的相对渗透率变化。但随着戊唑醇溶液浓度增加,抗性菌株的相对渗透率下降。在戊唑醇溶液浓度为4μg/mL和16μg/mL时,抗性菌株的相对渗透率显著增大。因此采用该方法可快速进行敏、抗性菌株的鉴定,从而为植物病原真菌的抗药性检测提供一种新方法。

The correlation between resistance of Fusarium graminearum to tebuconazole and relative leakage was studied by comparing changes of relative leakage between the sensitive and resistant isolates at different concontrations of tebuconazole. The result showed that tebuconazole at 1μg/mL and 0.045 1μg/mL （ECs0）, the change values of relative leakage of susceptible isolate were obviously bigger than that of resistant isolates. However, with the tebuconazole concentration increased, relative leakage of resistant isolates were increased. Under the treatment of tebuconazole with the concentration of 4μg/mL or 16 μg/mL, the change values of relative leakage of resistant isolates were increased significantly. Therefore, a new method by which resistant and susceptible isolates could be identified quickly, was provided for detection of resistance of plant pathogenic fungi.