肠道病毒感染与儿童Ⅰ型糖尿病关系的研究

A study on the relationship between type Ⅰ diabetes mellitus and enterovirus infection

目的 探讨儿童Ⅰ型糖尿病与肠道病毒 (EV)感染 ,特别是柯萨奇B组病毒 (CVB)感染的关系。方法 采用RT PCR方法 ,对 45例糖尿病患儿血中EV RNA进行检测 ,用微量中和试验方法 ,对糖尿病患儿和正常小儿血清中抗CVB中和抗体水平进行测定 ,同时用ELISA方法 ,测定CVB特异性IgM。结果 (1)糖尿病组 45例患儿中EV RNA阳性者为 17例 ,(39% ) ,对照组 5 1名儿童中阳性者为 3名 (6 % )。 (2 )检测 35例糖尿病患儿CVB IgM阳性者为 14例 (40 % ) ,40名对照组儿童中阳性者2名 (5 % )。 (3) 4 0例糖尿病患儿的CVB中和抗体检出为 32例 (80 % ) ,其中抗体异常者为 18例(5 6 % ) ;对照组CVB中和抗体检出为 40例 (82 % ) ;CVB感染型别分别以CVBB4 ,CVBB3 为多。结论儿童Ⅰ型糖尿病发病与肠道病毒感染 ,特别是柯萨奇B组病毒感染有关。

Objective To study the relationship between type Ⅰ diabetes mellitus and enterovirus (EV) infection. Methods EV RNA was detected in blood by reverse transcription polymerase chain reaction (RT PCR). Anti coxsackievirus B (CVB) neutralizing antibodies were detected by using micro neutralization test. At the same time, CVB specific IgM antibody was detected with ELISA. Results EV RNA was detected in 39%(17/45) of the patients and in 6%(3/51) of normal control group. Neutralizing antibody to CVB was found positive in 80%(32/40) of diabetes patients and in 82%(40/51)of normal controls, respectively. Abnormal neutralizing antibodies were found in 56%(18/32) of the patients. CVB specific IgM antibody was positive in 40% (14/35) and 5% (2/40) of patients group and normal group. Most of the CVB prositive individuals in both groups were infected with CVB3 or CVB4. Conclusion The results of this study suggest that there may be a significant correlation between type Ⅰ diabetes mellitus and EV infection.