ELPs-PDOR融合基因表达条件的优化

Optimization of Cultivation Conditions of ELPs-PDOR Fusion Gene

利用均匀设计法和二次多项式逐步回归,对重组大肠杆菌生产类弹性蛋白多肽-1,3-丙二醇氧化还原酶(ELPs-PDOR)重组蛋白的培养条件进行优化.结果表明:在装液量为30%,诱导剂浓度为6.3mmol·L-1,诱导温度为30℃,诱导时间为2h的优化条件下进行培养,重组菌融合蛋白表达量是原始表达量的3.3倍,酶活力提高到10.84mkat·L-1,提高2.1倍.

The optimal parameters of cultivation condition of eastin-like polypeptides-1,3-propanediol oxidoreductase（ELPs-PDOR） by recombinant Escherichia coli were obtained through uniform-design and two polynomial stepwise regression.The optimal conditions of experiment were as follows： the medium volume was 30%,the induction concentration was 6.3 mmol·L-1,the cultural temperature was 30 ℃ and the induction temperature was 2 h.On the basis of this,the yield of ELPs-PDOR is 3.3 times higher than the primitive expression,and the enzymatic activity of PDOR is 10.84 mkat·L-1 which is 2.1 times of the enzymatic activity of primitive expression.