接种于眼内腔可溶性抗原的释放和存留模式

The patterns of antigen release and local retention following intraocular compartment inoculation of soluble antigen

目的 确立前房和玻璃体腔接种可溶性抗原后,其抗原释放和局部存留的模式。方法 分别将99mTc和125I标记的人血清白蛋白接种于兔眼前房和玻璃体腔。使用SPECT和放射免疫测定技术检测标记抗原在眼内腔短期和长期的存留和释放模式。结果 接种于眼内腔的99mTc标记抗原,在短时间内部分进入血流,大部分存留于眼内腔。接种于前房的125I标记抗原在7～11天后外周血达到高峰,34～36天消失。接种于玻璃体腔的125I标记抗原在17～24天后达到高峰,60～65天消失。结论 眼内腔对抗原的储集及其缓慢的释放模式,为眼免疫偏离的诱导提供了必要的条件。

ObjectiveSoluble antigens such as bovine serum albumin (BSA) injected into the intraocular compartments evoke a deviant systemic immune response characterized by a selective deficiency of delayed type hypersensitivity (DTH).It has been known that the deviant immunity which follows intraocular inoculation is related to the uniqueness of the route of antigen administration.The purpose of this study is to observe the kinetics of antigen retention and release following inoculations of*' 99m technetium( 99m Tc) and 125 iodine( 125 I)*!labeled human serum albumin (HSA) into the anterior chamber and the vitreous cavity.Methods 99m Tc and 125 I labeled HAS as soluble antigen was inoculated into the anterior chamber and vitreous cavity of rabbits,respectively.Local and systemic metabolic pathways of 99m Tc labeled HSA were topographically imaged within 48*!h by using single photon emission computed tomoscopy (SPECT)technique.After intraocular inoculation of 125 I labeled HSA,labeled antigen from peripheral blood was dynamically analyzed for 70 d by radioimmunoassay.ResultsAfter the inoculation of 99m Tc labeled antigen into anterior chamber or vitreous cavity,part of labeled antigen was released into the blood stream in a short time,but most of it retained in intraocular compartments.When 125 I labeled antigen was injected into anterior chamber,labeled antigen in peripheral blood reached its peak between 7～11*!d and disappeared after 34～36 d. When 125 I labeled antigen was injected into vitreous cavity,labeled antigen in peripheral blood reached its peak between 17～24*!d and disappeared after 60～65*!d.ConclusionsIntraocular compartments function as antigen depots.Both the slow release of antigen from intraocular compartments into the blood stream and the unique microenvironment of intraocular compartments contribute to the induction of ocular immune deviation.