灵长目前房相关免疫偏离的诱导和维持不需要脾脏的完整性

Induction and maintenance of anterior chamber associated immune deviation don’t require an intact,functional spleen in primates

目的 观察脾脏在灵长目前房相关免疫偏离(ACAID)诱导和维持中的作用。方法 按照经典方法在猴眼建立ACAID模型。在前房抗原接种前切除脾脏。通过观察对抗原特异性迟发型超敏反应(DTH)的抑制来确定ACAID的诱导和维持。结果 在单纯脾脏切除组动物均显示阳性DTH反应;但在单纯前房抗原接种组、脾脏切除加前房抗原接种组均显示阴性DTH反应,且维持时间无显著差异。结论 灵长目ACAID的诱导和维持不需要功能性脾脏的存在。实验为研究人眼的免疫调控机制奠定了基础。

ObjectiveTo investigate the role of spleen in the induction and maintenance of anterior chamber associated immune deviation (ACAID) in primates.MethodsA soluble antigen,bovine serum albumin(BSA) or normal saline was inoculated into the anterior chamber of cynomolgus monkeys with sham splenectomy and splenectomy.Recipient animals were immunized with BSA and complete Freund’s adjuvant.Delayed type hypersensitivity(DTH) was assessed by skin challenge.The maintenance time of deviant immune response was evaluated in the fixed interval.Results Positive DTH reactions were induced in the control animals only with splenectomy.Antigen inoculated animals with sham splenectomy showed negative DTH reaction.Antigen inoculated animals with splenectomy also showed negative DTH reaction.In addition,the maintenance time of ACAID was not significantly different between the sham splenectomized and splenectomized monkeys.Conclusions Monkeys resemble mice in displaying ACAID when they first encounter an antigen via the anterior chamber,but the induction and maintenance of ACAID don’t require intact,functional spleen.We believe that the function of spleen may be replaced by that of other lymphatic organs as the evolution results of primates including human.The findings suggest that human eyes have more complex mechanisms of immune privilege.Primate eyes offer another model to further explore the mechanisms of ACAID for human eyes.