竹黄菌液体培养下产竹红菌素的研究

Study on fermentation hypocrellin by Shiraia bambusicola in submerged cultures

先对不同产地采集的竹黄菌进行筛选,得到优产竹红菌素的菌株,然后采用单因子和3因素3水平正交试验法对竹红菌素液体发酵条件进行优化,在优化培养基的基础上,选用不同浓度的Cr3+、Fe3+、Cu2+和Ca2+对竹红菌素进行离子调控研究。结果表明:从休宁所采集的菌株不仅生长速度最快,发酵所产的竹红菌素含量也最高;竹红菌素最佳发酵碳源是葡萄糖,最佳发酵氮源是硝酸钠,最佳培养基组合为2%葡萄糖,0.2%硝酸钠,pH7.5;Cr3+和Fe3+浓度为0.005%时竹红菌素含量均最高;0.05%的Ca2+最有利于竹红菌素的分泌;Cu2+为0.03%时竹红菌素含量达到最大值。

Shiraia bambusicola from different regions were firstly assayed for their hypocrellin production,thus the high performance strains of Shiraia bambusicola were obtained.Then the culture media was optimized by one-factor-at-a-time and three factors and three levels of orthogonal matrix methods.Subsequently,the optimized conditions were then assayed for their effects on accumulation of hypocrellin in the presence of different levels of Cr3＋、Fe3＋、Cu2＋ and Ca2＋.The results showed that the strains collected from Xiuning grew faster and produced relatively high amount of hypocrellin than those from other regions.The most suitable carbon sources and nitrogen sources were glucose and NaNO3;the growing medium consisting of glucose（2%）,NaNO3（0.2%）,KH2PO4（0.01%）,MgSO4（0.05%）with pH level at 7.5 yielded the maximum production rate of hypocrellin.This could be further increased following the addition of Cr3＋、Fe3＋、Cu2＋ and Ca2＋.Addition of Cr3＋ and Fe3＋ at the concentration of 0.005% both stimulated the hypocrellin production and the biosynthesis;Ca2＋ of 0.05% was most suitable for the hypocrellin production;addition of Cu2＋ of 0.03%,the hypocrellin production rate reached the maximum level.