舒巴坦对产β-内酰胺酶菌株的抑酶增效作用

Stability of pipercillin/sulbactam to 33 strains gram negative bacillus of β-lactamases from isolates

目的 比较哌拉西林、哌拉西林 /舒巴坦和氨苄西林 /舒巴坦对产 β 内酰胺酶细菌的抑酶增效作用。方法 以生物法测定 3 3株临床分离的革兰阴性杆菌和 5株标准菌株产 β 内酰胺酶对抗生素的相对水解率 ,抑酶保护率。结果 β 内酰胺酶对哌拉西林 /舒巴坦各配比的相对水解率明显低于单独使用哌拉西林 (P <0 .0 1) ,结果还表明哌拉西林 /舒巴坦 ( 2∶1)与氨苄西林 /舒巴坦相同 (P >0 .0 5 )。哌拉西林 /舒巴坦不同配比间 ,随舒巴坦比例增大相对水解率降低 ,抑酶保护率提高 ,1∶1与 2∶1配比间无统计学意义 ,用中位数做显著性测试 ,1∶1与 4∶1配比间差异有显著性 ,P <0 .0 1。结论舒巴坦与哌拉西林合用提高了哌拉西林对产 β 内酰胺酶细菌的抗菌活性 ,预示将会有更好的临床疗效和应用前景。

Objective To compare the stability of pipercillin, pipercillin/sulbactam and ampicillin/sulbactam to preparation of crude β lactamses due to gram negative bacilli from 38 strains (33 strans from clinic). Methods The relative hydrolysis rate and prevention rate were determined by bioassay method. Results The relative hydrolysis rate of piperacillin/sulbactam was lower than that of piperacillin. The rate of piperacillin/sulbactam (1∶1) was higher than that of piperacillin/sulbactam (4∶1), and the rate of piperacillin/sulbactam (2∶1) was close to that of ampicillin/sulbactam. Conclusion Combined piperacillin/sulbactam was more stable to β lactamases produced by gram negative bacilli than piperaillin.