大黄乙醇提取物的鸡体内抗新城疫病毒试验

Anti-viral Effect of Rhubarb Ethanol Extract on Newcastle Disease Virus to Chickens in Vivo

以雏鸡作为试验鸡,在NDVIV系病毒感染的不同时间段(感染前5～6h、感染后5～6h、感染后18～20h),以3个不同剂量的大黄乙醇提取物经皮下注射和口服给药,进行体内抗病毒试验,以脾脏中NDVIV系病毒相对含量作为抗病毒作用效果。试验结果表明,在NDVIV系病毒感染前5～6h及感染后5～6h,以3个不同剂量的大黄乙醇提取物处理,脾脏中NDVIV系病毒相对含量都明显下降;而在NDVIV系病毒感染后18～20h,以3个不同剂量的大黄乙醇提取物处理,脾脏中NDVIV系病毒相对含量下降不明显。在NDVIV系病毒感染的适宜时间,以适宜剂量大黄乙醇提取物进行防治具有一定疗效;大黄乙醇提取物在试验鸡体内抵抗NDVIV系病毒感染与给药剂量及给药时机有密切关系;大黄乙醇提取物5.0mL·kg-1·次-1大剂量组对试验鸡是安全的。

Antiviral experiment in vivo was did in chicks with rhubarb ethanol extracts of three different dose by subcutaneous injection and oral administration in different time periods of the NDV IV strain virus infection （5~6 h before infection,5 ～6 h post infection, 18～20 h post infection）, and antiviral effect was evaluated by relative amounts of NDV IV strain virus in the spleen. The test results showed that relative contents of NDV IV strain virus in spleen decreased obviously, the chick were treated with three different doses of rhubarb ethanol extract in 5～6 h before infection and 5～6 h post infection with the NDV IV strain virus; while in 18～20 h post infection by NDV IV strain virus, the chick were treated with three different doses of rhubarb ethanol extract, relative contents of NDV IV strain virus in spleen did not decreased obviously. The experimental results showed that the prevention had definite curative effect in infection suitable time of the NDV IV strain virus with suitable dose of rhubarb ethanol extract, and the curative effect against infection of NDV IV strain virus was connected closely with drug dosage and dosing time with rhubarb ethanol extract in experimental chickens, and rhubarb ethanol extract （5.0 mL·kg-1 body weight, large dose group） was safe to experimental chicken.