苹果梨果实着色相关PAL基因片段克隆及表达分析

Cloning and expression analysis about fruit coloring related Phenylalanine Ammonia-lyase gene fragment from Pingguoli

以苹果梨果皮为试材,克隆了苹果梨果实着色相关PAL基因片段,GenBank登录号为JN120855。结果表明:该基因片段长度为407bp,与鸭梨PAL基因序列一致性达97%,与鸭梨氨基酸序列同源性最高,酶切位点分析表明,该PAL基因序列含有常用的限制性内切酶AccI的识别位点。半定量RT-PCR分析表明,随着果实的成熟,套袋果与未套袋果PAL基因的表达量都不断增加,但套袋果在果实成熟期的表达量明显高于未套袋果。

Make the Pingguoli peel as the material,coloring related PAL（fragment） was cloned,accession number：JN120855.The results showed that the fragment includes 407 basepair,the consistency between fragment and Yali pear PAL gene is 97%,and the amino acid sequence homology is the highest,restriction analysis showed that there was a commonly used restriction enzyme site for AccI.The semi-quantitative RT-PCR technique showed that as fruit mature,expression of PAL gene is increasing constantly about bagged fruit and no bagged fruit,but expression of the bagged fruit is obviously higher than no bagged fruit in the fruit mature.