黑松和马尾松苗受松材线虫侵染后部分化学物质的响应

Physiological Response of Pinus thunbergii and Pinus massoniana to Bursaphelenchus xylophilus Infection

为研究松材线虫侵染对寄主植物生理生化物质代谢的影响,以黑松和马尾松4 5年生苗为实验材料,测定了接种松材线虫对2种寄主植物的营养物质和次生代谢物质含量的变化及其规律。结果显示:接种松材线虫初期,黑松和马尾松的总糖含量均较高,随接种时间的延长,总糖含量呈不断下降趋势;黑松的可溶性糖含量一直降低并明显低于对照,马尾松的可溶性糖含量在侵染前期与对照相比变化不明显,而15 d后快速降低;黑松和马尾松的可溶性蛋白含量侵染前期均低于对照,后升高,再降低;黑松的单宁含量明显高于对照,马尾松的单宁含量接种第1天略低于对照,第3天后开始升高;黑松和马尾松的总酚含量均在侵染前期高于对照,而后降低。这些物质的变化趋势显示松材线虫侵染不同寄主植物的生理反应。

In order to study the effects of Bursaphelenchus xylophilus infection on the nutritional chemicals and secondary metabolites of the host plants,the total sugars,reducing sugar,soluble sugar,soluble proteins,tannins and total phenol in stem were assayed in two host plants Pinus thunbergii and P.massoniana inoculated with B.xylophilus.The results indicated that the total sugar concentration of both P.thunbergii and P.massoniana increased after inoculating and then decreased gradually with the extending of the attack time.The soluble sugar content of P.thunbergii decreased obviously,while that of P.massoniana changed slightly until 15 days and then decreased.The soluble protein content of both host plants decreased at first,then increased and decreased again.The tannin content of P.thunbergii increased all along with infecting time,whereas that of P.massoniana decreased in the first day and increased until 30 days.The total phenol content of P.thunbergii increased in the first 7 days and then decreased in the following days,but that of P.massoniana continued to increase after inoculating,and showed significant difference from the third day on.The changing tendency of above substances interpreted the physiopathology response of different host plant infected with B.xylophilus.