马占相思优树组培快繁技术研究

Tissue Culture Technique of Acacia mangium Elite Trees

相思（Acacia Mill.）类树种（俗称相思树）属含羞草科（Mimosaceae）金合欢属（Acacia Mill.）,在我国广东、海南、广西、福建、云南和江西等地均有栽培。相思树植株高大挺拔,根系发达具根瘤,具有耐干旱贫瘠及抗风的特性,是水土保持及改良土壤的优良树种;同时,相思树还是优良的用材树种,

Taking Acacia mangium elite trees as explants,the tissue culture technique system for dormant bud of 3-5 year-old elite trees was established.The system includes the germination-inducing of the dormant buds,the multiplying of shoots,the rooting of adventitious shoots,and the pre-treating and transplanting of seedlings.The medium MS ＋ Sucrose 30 g·L^-1 ＋ BA 0.5 mg·L^-1 ＋ NAA 0.1 mg·L^-1 was used for inducing the dormant buds;the medium MS ＋ BA 1.0 mg·L^-1 ＋ NAA 0.05 mg·L^-1 was used for multiplying;while the medium 1/2 MS ＋ IBA 2.0 mg·L^-1 ＋ NAA 0.5 mg·L^-1 was used for rooting.The results revealed that it was viable to producing field seedlings with micropropagation.Although the branches with dormant buds harbored many kinds of microbes and the adventitious shoots were not easy to root,20% to 30% healthy germination could be yielded and the rooting rate of adventitious shoots could be higher than 85%.Pretreated with ABT powder（rooting hormone）,both the rooting rate and survival rate of adventitious shoots were nearly 100%.