氯霉素印迹聚合物膜的制备及其吸附性能研究

Preparation of chloramphenicol imprinted polymer membranes and its adsorption characteristics

利用分子印迹技术紫外光引发原位聚合的方法,以氯霉素为模板分子,以4-乙烯基吡啶为功能单体,以聚丙烯无纺布微孔滤膜为支撑膜,制备了具有选择识别性的氯霉素印迹聚合物膜;采用紫外光谱分析研究了模板分子和功能单体之间的作用,使用扫描电镜观察了线性聚合物添加剂PEG用量对膜的表面形貌的影响,并对印迹聚合物膜进行了平衡吸附和特异性吸附实验,研究其识别能力.结果表明:氯霉素和4-乙烯基吡啶之间形成了氢键;PEG质量分数为16%时膜表面形态最佳,孔结构清晰均匀;本实验所合成的印迹膜对氯霉素分子表现出了良好的特异性识别能力,吸附量达到22.83μmol/g.

By using chloramphenicol as template,4-vinylpyridine as functional monomer and porous polypropylene non-woven as the support membrane,chloramphenicol imprinted polymer membranes with predominant recognition and selectivity are prepared through in-situ polymerization by UV irradiation.The interaction between chloramphenicol and functional monomer is verified by UV-visible spectroscopy.The morphologies of chloramphenicol imprinted polymer membranes with different PEG dosage are observed by the scanning electron microscopy（SEM）.The binding characteristics of the imprinted membranes are studied by the equilibrium binding experiments and specific adsorption experiments.The results show that hydrogen bonding is formed between template molecule and functional monomer.When the mass fraction of PEG is 16%,the prepared imprinted polymer membranes have perfect morphologies and uniform pore structure.It is found that the imprinted polymer membranes show good recognition characteristics to chloramphenicol and the max binding amount reached 22.83 μmol/g.