复方中药(苦黄汤)对大鼠原位肝移植急性排斥反应的影响

Effect of Compound Traditional Chinese Medicine Kuhuang Decoction on Acute Rejection after Orthotopic Liver Transplantation in Rats

目的观察复方中药(苦黄汤)对大鼠原位肝移植急性排斥反应(AR)的影响。方法建立Wistar-SD原位肝移植AR模型。将制备的80只大鼠模型按随机数字表法分为:生理盐水组(A组)、苦黄汤组(B组)、环孢素A组(C组)和苦黄汤+环孢素A组(D组),每组20只。各组受体大鼠术前第1天至术后30d分别灌胃给药(1次.d-1):A组仅给予生理盐水2mL.d-1,B组给予苦黄汤2mL.d-1,C组给予环孢素A 10mg.kg-1.d-1,D组给予苦黄汤1mL.d-1+环孢素A 5mg.kg-1.d-1。术后第7天,每组各处死10只大鼠,取腔静脉血检测丙氨酸转氨酶(AST)及天冬氨酸转氨酶(ALT)的值,光镜下观察肝脏组织病理改变。每组余下的大鼠进行生存期观察。结果其他各用药组与A组相比:大鼠术后的生存时间明显延长(P<0.05),以D组大鼠为最长;大鼠的AST及ALT的值明显下降(P<0.05),以D组最为明显;排斥反应指数明显降低(P<0.05),以D组最为显著。结论苦黄汤具有抑制大鼠原位肝移植AR的作用,且与环孢素A具有协同作用。

Objective To observe the effect of compound traditional Chinese medicine Kuhuang decoction on acute rejection（AR） after orthotropic liver transplantation（OLT） in rats.Methods The model of OLT was established in 80 rats（Wistar donors and recipients）.Liver recipients were,respectively,administered intragastrically with 2 mL·d-1normal saline（group A）,2 mL·d-1 Kuhung decoction（group B）,10 mg·kg-1·d-1cyclosporine A（group C） and 1 mL·d-1 Kuhuang decoction plus 5 mg·kg-1·d-1cyclosporine A（group D） from 1 day before operation to 1 month after operation once per day.Ten recipients in each group were sacrificed on postoperative day 7.Liver tissue was collected to observe the intensity of acute rejection and vena cava blood was taken to measure the levels of aspartate aminotransferase（AST） and alanine aminotransferase（ALT）.The remaining rats were fed to record the survival time.Results Compared with group A,mean survival time significantly increased,and AST,ALT and rejection activity index obviously decreased in other groups（all P0.05）,especially in group D.Conclusion Compound traditional Chinese medicine Kuhuang decoction can inhibit acute rejection after OLT and has a synergistic effect with cyclosporine A.