摘要
目的:利用纳米技术和基因干扰技术设计并合成携载survivin siRNA的纳米载体,探讨survivins iRNA纳米微粒对人胰腺癌细胞BXPC-3增殖和凋亡的影响。方法:体外培养人胰腺癌BXPC-3细胞,将BXPC-3细胞随机分为4组:生理盐水组、不含基因的纳米微粒组、survivin siRNA组和s urvivins iRNA纳米微粒组。RT-PCR检测survivin mRNA的表达;Wes tern blot法检测s urvivin蛋白的表达;流式细胞仪检测细胞凋亡情况;MTT法检测细胞增殖情况。结果:细胞培养72 h后,survivin siRNA纳米微粒组细胞的survivin mRNA和蛋白表达均低于其他3组(P<0.05)。survivin siRNA纳米微粒组细胞增殖明显受抑制,生长缓慢,而细胞凋亡率高于其他3组(P<0.05)。结论:survivin siRNA纳米微粒能够有效减少胰腺癌BXPC-3细胞survivin mRNA和蛋白的表达,提高肿瘤细胞的凋亡,显著抑制BX-PC-3细胞的增殖。
Objective: To used nanotechnology and gene interference to block survivin expression in pancreatic carcinoma cell and to study the inhibitory effect of small interference RNA targeting survivin nanospheres on the proliferation and apoptosis of human pancreatic cancer BXPC-3 cells.Methods: Human pancreatic cancer BXPC-3 cells cultured in vitro were assigned into four groups: saline,gene-free nanospheres,siRNA-survivin and siRNA-survivin nanospheres groups.Survivin mRNA expression was detected by RT-PCR;Survivin protein expression was detected by Western blot;Apoptosis of BXPC-3 cells was determined by flow cytometry;BXPC-3 cells growth was examined by MTT.Results: At 72 hours after treatment,both survivin mRNA expression and survivin protein expression in the siRNA-survivin nanospheres group were significantly less than other three groups(P0.05).BXPC-3 cell growth was remarkably inhibited in the siRNA-survivin nanospheres group,but apoptotic rate was significantly greater than other three groups(P0.05).Conclusion: siRNA-survivin nanospheres can specifically reduce both survivin mRNA and survivin protein expressions in human pancreatic cancer BXPC-3 cells,significantly increase tumor cell apoptosis,and remarkably inhibit BXPC-3 cell proliferation.
出处
《中国现代普通外科进展》
CAS
2012年第4期253-257,共5页
Chinese Journal of Current Advances in General Surgery
基金
中国博士后科学基金资助项目(2011M500697)