MPAIA日本血吸虫抗体检测中室内质量控制方法探讨

Discussion of indoor quality control method of MPAIA antibody test of Schistosoma japonicum

目的探讨科学有效的室内质量控制方法,使磁微粒分离酶联免疫法(Magnetic Particle Antibody Immunoassay,MPAIA)用于血吸虫抗体检测的结果真实可靠。方法把即刻法、Z分数图及Westgard多规则质控方法相结合,开展MPAIA血吸虫抗体检测室内质量控制工作,所得数据建立数据库,并进行统计分析。结果即刻法从第3次试验开始就进入质控状态,20次试验后转入Z分数常规质控,Westgard多规则质控方法能帮助分析误差的类型。在第3-40批次试验中,共完成186份患者样本检测,有2个批次9份患者样本试验结果因质控品失控,需重新进行试验分析。其他在控批次有177份患者样本结果均可接受。结论试验中应用即刻法、Z分数图、Westgard多规则质控方法进行质控分析能较好地完成MPAIA血吸虫抗体检测常规质量控制工作。

Objective To explore the scientific and effective indoor quality control method to ensure the results of Magnetic Particle Antibody Immunoassay（MPAIA） antibody test of Schistosoma japonicum reliable.Methods The MPAIA antibody test indoor quality control work was performed with the combination of the crubbs method,Z-scores figure and Westgard multi-rules quality control method.Results The crubbs test entered the quality control condition state from the third times,after 20 times,the test switched to Z-scores conventional quality control,and the Westgard multi-rules quality control method could help analyze the type of errors.From the third to fortieth test,186 samples were completed and the tests of 9 samples among 2 batches needed to be re-done because of the failure of quality control.The test results of other 177 samples were acceptable.Conclusion The combination of the crubbs method,Z-scores figure and Westgard multi-rules quality control method can effectively accomplish the indoor quality control of MPAIA antibody test of Schistosoma japonicum.